for molecular biology. Top and bottom reads are. D. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Access this by going to. 5032338. 0025 OD from 0. Product Compare 0. 00. No additional manuals are available. x. SoftMax Pro GxP Software version 5. Four wash/rinse bottles are included and configurable for both 96- and 384. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. 0 . Plate Background OD, which was used for subsequent tests, was measured as follows. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 01. HeLa. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. 2b,c. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. When the user applies a set temperature of 37°C, the top is heated to 37. The plate reader can measure multiple readouts, including UV and. We would like to show you a description here but the site won’t allow us. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. 2 OD at 200 nm; this decreased to 0. 2. Can read 96 well plates (optional 384 well). Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Supersedes 1. Published Dec 16, 2020 | Updated Dec 06, 2021. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 1. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. x. Temperature control: 4°C above ambient to 45°C. x. The Versamax Microplate Reader is built around a grating monochromator. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Pick the best speed, sensitivity, and price for your needs. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices' microplate readers. * Note: Programming the washer with a final aspirate height of 4. 7°C (gradient heating design). The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Washer maintenance kit: 2 X T 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Align the two slots on the bottle holder with the two pegs on the rail. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Reader Documentation Version No. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Answer. Three low volume 24-well bottom slides. #23225). style. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. UV absorbance detection. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. Description. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Step 3: Click “Settings” and a screen will appear (Figure 1). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. It also integrates with the. It offers extremely low absorbance background, excellent. 96- or 384-well microplate compatibility. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Step 2: Launch SoftMax Pro Software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Supersedes 1. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Consequently, FLIPR has been used. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Place the microplate in the drawer. Important things to consider when purchasing a multimode microplate reader. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. #E010) Pyroplate 96-well microplate (ACC cat. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. The soil was amended with either 1. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. #CA961) Methods. 1 mL/well of media. SoftMax Pro GxP Software version 5. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 984. They can be read by most microplate readers. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Published Dec 16, 2020 | Updated Dec 16, 2020. 5 ng per well. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 315 can't find zero order. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The VICTOR Nivo. In addition, the iD5 reader measures TRF and FP and. Microplate Spectrophotometers. Learn how you can utilize microplate readers to drive landmark discoveries. The procedures in this protocol support the 96-well reaction plate. 96- and 384-well microplates. What are the dimensions of the plate? Code. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. 50/plate. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Contact our support team with your questions regarding Molecular Devices products and applications. minutes and begin reading microplates with seven mouse clicks. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. 190. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Three kits covering a wide range of dsDNA concentrations. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Compact designs make these instruments. We would like to show you a description here but the site won’t allow us. (96- or 384-well) simultaneously and with high temporal resolution. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. All trademarks used herein are the property of. Features. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. and the A1 corner of the plate adapter (Figure 1-2). Bowman Dispensers. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Compare. Fluorescence Microplate Reader Comparison Chart. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Well scanning With well scanning, multiple points within each well can be read, providing a high. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. 4 decades . 5 µL of each sample was pipetted into a 96-well plate for PCR. Published Dec 16,. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Service Contracts, Preventative Maintenance and. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Other. In SoftMax Pro, Dual Read mode was selected. 5 ng per well. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 20 µL (96-well plates) or 10 µL (384. Compare specifications and request a quote from leading suppliers. This reader's dual monochromators for. This can increase throughput and efficiency, thus saving time and space in the lab. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. Measurements for every well are automatically tracked over time. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Selects either 96-well or 384-well plate for a stand-alone read. 96-well plates - Filter plates with 0. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. Published Dec 16, 2020 | Updated Nov 07, 2022. Top or bottom detection is available with a simple click of a button. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. No accessories are needed and SoftMax® Pro Software. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. M2. President of Molecular Devices. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Optional 12-way manifold) 5032336. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. Luminescence applications and assays. 313 reference gain check fail. No. More Product Information. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Instrument pricing. Suggested plates are black walled. Molecular Devices has provided scientists with . These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 1. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. Absorbance equation. Our microplate. High tolerance for contaminants. Materials. Shaking: Linear, orbital, and double orbital. Click Add to create the plate. Wavelength(s):. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Usage/Application: Lab. Click SoftMax Pro Software User Guide. #ABS Plus) Cell plating and treatment prior to assay. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. These robust readers have been placed in labs from Antarctica to the. REF (SpectraMax. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. The 6-well plates were incubated at 37ºC for 9 days before picking. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. . ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Molecular Devices (UK) Ltd. Two high-energy xenon flash lamps: Reading capabilities. Two different agonists, acetylcholine and carbachol, were tested. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . perfect for kinetic readings involving growth curves at 5 seconds per plate. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. Published Dec 16, 2020 | Updated Dec 16,. The drawing shows a standard microplate of 15 mm height covered with a lid. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Some will only support one well density (e. Cayman Chemical. 100 fmol 1536-well (8 µL) 0. 3% in white 96- and 384. These microplates have a corner notch for use in standard plate readers. Microplate optimization and read height optimization were performed for best results. . 0200-6263. Specifications subject to change without notice. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Polypropylene deep well plates, round bottom. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. Compare this item. the use of a reader calibrated with primary NIST and NMI standards. Self-righting base, up to 40 degrees past center without dispenser. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. Recertification service. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). M2. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. as well as all other Molecular Devices' microplate readers. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Making it possible to perform kinetic analysis of solid. Absorbance Linearity: 2. 1 OD at 230 nm. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The CellTiter-Glo assay was used to measure ATP. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 96-, 384-, and 1536-well microplates. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. 25, 3. Temperature control range from Ambient +4º to 50ºC. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. HeLa. Supplier: Molecular Devices. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Microplates, cuvettes (via adapter) Detectors. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 000 – 4. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. 001 ABS (displayed), 0. It also integrates with the powerful SoftMax Pro Software for. 2. These plates are compatible with standard fluorescence microplate readers and are offered in a. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. x. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. QC tools and IQOQ services. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The VersaMax reads 96-well microplates. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 4 (Molecular Devices cat. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. VMax . Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Software Validation. The IC50s by cell rounding and LysoTracker. 5°C at 37°C. Multimode microplate reader equipped with latest detections like fluorescence,. Multimodal plate reader: Yes: 25 mm: Yes—Max. absorbance microplate reader GEA. Another consideration, says Amouretti, is plate format. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. TNF standard curve. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. a white 96-well half-area microplate (Corning cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 100 fmol 384-well (75 µL) 0. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 6. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. 057, depending on lot number. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. x. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 6-384 (96- and 384-well with pipetting) 96-well microplates. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). (Molecular Devices cat. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 1. We would like to show you a description here but the site won’t allow us. 340 PC384. Source plate capacity; 1 x plates. C. 305 reference level saturation. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. Step 1: Capture antibody binds to ELISA plate wells. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. Browse for different fluorescent readers below and compare across different instrument suppliers. Inconsistent results: cannot determine whole well confluence – well after well. M5.